Find a recent article from a scientific journal (the ACS Journals are available online from on-campus) about measuring the interaction between a protein and a ligand. Make a copy of the article to annotate and hand in with your answers. For each question, indicate where in the article you found your answer.

Note the following about the article:

• The equation used to analyze the data.
• Whether bound protein or bound ligand was measured.
• Whether cooperative interactions were encountered.
• The specificity of interaction between protein and ligand.
• Whether the binding interaction is considered to be high-affinity.

Also find an article measuring constants associated with enzyme kinetics. Identify about the experiments:

• what kinetic model was used to analyze the data.
• if the experiment measured product formed or reactant consumed.
• if the pH dependence of the reaction was measured, or how the pH of the reaction was selected.
• the significance of the measured kinetic constants.
• whether covalent modifications of the enzyme are necessary for the measured activity.
• if the enzyme is allosterically controlled.

These articles should be studied in lieu of class attendance. The articles with associated answers will be due in class on Wednesday, September 26.