Shimadzu UV-Vis

The Shimadzu UV-vis is a scanning spectrometer that can cover the 200-1100 nm range. Before using the instrument, it should be turned on using the power switch in the back and allowed to run through its diagnostic cycle and stabilize (should take about 20 min for the lamps to stabilize).

The native mode of the spectrometer is a single wavelength measurement. To scan a spectrum, an optional program pack is needed. The program pack card should be placed in the slot in the front of the spectrometer.

After the machine has finished its diagnostic cycle, you should see a screen with two choices. Press 2 to choose the Option Program Pack (scanning). Choosing 1 will allow you to scan in single wavelength mode.

The next step is to set the parameters. You will need to change the scanning range, and scan speed. To change the scanning range, press 2. It will then ask for you to input the range (as shown below). You need to enter the higher wavelenth FIRST. When finished, press 4 to change the scan speed. You will want the speed to be FAST.

When finished, you can insert the cuvette with your solvent (no sample, just the blank) into the spectrometer.

Make sure the polished (unfrosted) sides of the cuvette are lined up with the light path. Close the lid before scanning.

To scan the baseline, press the F1 button (right under the BaseCorr option on the screen). Wait for the scan to finish before removing the cuvette. Remove the cuvette and add the sample. Then place the cuvette back into the sample chamber.

Press the Start button to scan the sample.

When finished, the unscaled spectrum should be visible on the screen.

Choose Zoom to scale the spectrum. The machine will ask you to enter starting and ending values for the absorption and wavelength scales.

To print the spectrum, Press and hold the F4 button and then press the right arrow button.

To pick peaks, choose the Peak option on the screen. Press F4 and the right arrow button again to print.

Remember: Turn off the machine when you are done to extend the lamp life.

This tutorial was created by Michael R. Jordan at Oklahoma Baptist University. Reproduction by printing or photocopying for instructional use by educational institutions is allowed.